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Volume 31 #1

Contents

  1. Sensory properties of reward pattern affect food preferences in rats
  2. Probable role of p38 MAP-kinase in reception of infrared irradiation
  3. Investigation of Src-kinase inhibitor PP2 effects on modulation of slow sodium channels by ouabain
  4. Perimetric assessment of a blind zone margin at the extreme periphery of human temporal retina
  5. LogMAR for visual acuity is worse than horsepower for electric lamp
  6. Bright light induced freezing behavior in American cockroach, Periplaneta americana
  7. Analysis of human eye movements in the management of self-propelled chassis with video eye-tracking interface system
  8. Mechanisms of transduction of olfactory signals: the possible role of the contractive apparatus of the olfactory ciliae
  9. Architecture of a system for computer vision-based vehicle detection and classification under natural conditions
  10. Localization of a stereo camera equipped robot using poles with known location as landmarks

Investigation of Src-kinase inhibitor PP2 effects on modulation of slow sodium channels by ouabain

© 2017 Т. N. Shelykh, I. V. Rogachevsky, V. N. Moshkina, S. A. Podzorova, B. V. Krylov, V. B. Plakhova

Pavlov Institute of Physiology, RAS, 199034 Saint-Petersburg, Makarova emb., 6.

Received 03 Aug 2016

Possible involvement of Src-kinase in transduction of nociceptive signals evoked by binding of ouabain to the transducing site of Na+, К+-АТPase was investigated by patch-clamp method. Speci c Src-kinase inhibitor (PP2) introduced into intracellular solution prior to extracellular action of ouabain at low concentrations was demonstrated to totally block modulation of slow sodium channels NaV1.8 by ouabain: the value of effective charge transfer (Ze ) of Nav1.8 channels activation gating device after ouabain application was the same as in the control experiments. Src-kinase was thus shown to be involved as a series unit in transduction of the nociceptive signal from Na+, К+-АТPase to NaV1.8 channel.

Key words: nociception, patch-clamp method, slow sodium channels, cardiotonic steroids, Src-kinase, Na+, К+-ATPase

Cite: Shelykh Т. N., Rogachevsky I. V., Moshkina V. N., Podzorova S. A., Krylov B. V., Plakhova V. B. Issledovanie vliyaniya ingibitora src-kinazy pp2 na sposobnost uabaina modulirovat medlennye natrievye kanaly [Investigation of src-kinase inhibitor pp2 effects on modulation of slow sodium channels by ouabain]. Sensornye sistemy [Sensory systems]. 2017. V. 31(1). P. 16-21 (in Russian).

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