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Volume 30 #3

Contents

  1. The structural-functional organization of mammalian auditory cortex as the basis of acoustic information cortical processing. The structural organization
  2. Allocation of the characteristic features of in low-frequency envelope of the tone by the neurons in the midbrain auditory center of the frog
  3. Discrimination of sound signals with rippled spectra in the noises of different spectral compositions
  4. Temporary threshold shifts in naïve and experienced belugas
  5. The role of simpatic-epinephrine system in the changes of electrical activity of the brain hemisphere cortex and hypothalamus under vibration exposure
  6. Cyclic polypeptide PP-14 modulates the voltage sensitivity of slow sodium channels
  7. Training optimal Viola–Jones detectors using greedy algorithms for selecting control parameters with intermediate validation on each level
  8. Abnormal band in the circular dichroism spectrum of DNA cholesteric liquid-crystalline dispersions – an analytical criteriom for detection of coloured biologically active compounds

Cyclic polypeptide PP-14 modulates the voltage sensitivity of slow sodium channels

© 2016 V. B. Plakhova, I. V. Rogachevsky, Т. N. Shelykh, S. A. Podzorova, B. V. Krylov

Pavlov Institute of Physiology, Russian Academy of Sciences Saint-Petersburg, 199034 Makarova emb., 6

Received 04 Apr 2016

An ability of polypeptide PP-14 to modulate the excitability of nociceptive neuron membrane was investigated by patch- clamp method. The agent was demonstrated to decrease the voltage sensitivity of slow sodium channels NaV1.8, responsible for nociceptive signal coding. Extracellular application of PP-14 induced a significant decrease in the effective charge transfer (Zeff) of the activation gating machinery of NaV1.8 channels, which indicates that the polypeptide may produce analgesia. Nevertheless, the effect of PP-14 is less pronounced as compared to a sharp decrease in nociceptive neuron excitability induced by a more complex molecule of defensin NP-1 applied at subnanomolar concentrations. Quantum- chemical analysis of PP-14 and NP-1 steric structures made it possible to account for the relative efficacy of ligand- receptor binding of these polypeptides.

Key words: nociception, patch-clamp method, slow sodium channels, polypeptides, quantum-chemical calculations

Cite: Plakhova V. B., Rogachevsky I. V., Shelykh Т. N., Podzorova S. A., Krylov B. V. Tsiklicheskii polipeptid pp-14 moduliruet potentsialochuvstvitelnost medlennykh natrievykh kanalov [Cyclic polypeptide pp-14 modulates the voltage sensitivity of slow sodium channels]. Sensornye sistemy [Sensory systems]. 2016. V. 30(3). P. 234-240 (in Russian).

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