Volume 36 #2

Contents

1. Component N2pc in Studying Visual Attention
2. Conceptual Model of Sensor System Ontology with Event Information Processing Method
3. Regeneration of the Helix lucorum visual body in a 185-day orbital flight et the International Space Station
4. Multiplicatively Сlosed Spectral Models in Color Analysis
5. About Monitored Tomographic Reconstruction

The article presents the results of the space experiment (SE) “Regeneration-1” on the gastropod ground pulmonary mollusk Helix lucorum. The 101 Snails were divided into three experimental groups: a flight group (FG) and two control groups – a synchronized escort group (SG) and a vivarium group (VG). Eye tentacles were amputated in all snails 10–11 hours before the onset of SE. The FG snails were transported to the International Space Station in a special lightproof container on the Soyuz TMA-19 manned spacecraft. The duration of the exposure in the orbital flight was 185 days and 22 hours. The SG snails were kept in a similar flight container at a temperature close to the onboard one. VG snails were kept in spacious terrariums under normal light conditions and temperatures of 20–22C, and had free access to food and water. The state of vision of native and regenerated eyes was assessed by morphological parameters, total electrical activity of the retina and spike activity of the optic nerve to single and paired light stimuli of different brightness and duration. After the completion of the experiment, regeneration of the eye tentacles with functioning eyes was observed in 75% of FG snails, 64% of SG snails and 94% of VG snails. The morphological and electrophysiological parameters of the regenerates of both flight and control snails were similar to each other and were noticeably inferior to the native eye tentacles during the 4-year period after the experiment was completed. Light deprivation did not affect the nature of the regeneration. In the regenerated optic nerve, there was no “off”– reaction to switching off the light stimulus.

Key words: mollusk, regeneration, vision, electroretinogram, weightlessness, International Space Station

DOI: 10.31857/S0235009222020044

References:

• Vorontsova M.A. Regeneratsiya organov u zhivotnykh [Regeneration of organs in animals]. M. 1949. 270 p. (in Russian).
• Vorontsova M.A., Liozner L.D. Bespoloye razmnozheniye i regeneratsiya [Asexual reproduction and regeneration]. M. 1957. 416 p. (in Russian).
• Gorgiladze G.I. Regeneratsionnaya sposobnost' u planariy i ulitok, eksponirovannykh v nevesomosti v orbital’nom polete na Mezhdunarodnoy kosmicheskoy stantsii [Regenerative capacity in planarians and snails exposed to zero gravity in orbital flight on the International Space Station]. Doklady akad. nauk. 2008. V. 421. № 1. P. 1–4 (in Russian).
• Gorgiladze G.I. Regeneratsiya organa zreniya v 163-sutochnom orbital’nom polete na Mezhdunarodnoy kosmicheskoy stantsii [Regeneration of the organ of vision in a 163-day orbital flight on the International Space Station]. Doklady akad. nauk. 2011. V. 440. № 4. P. 553–556 (in Russian).
• Gorgiladze G.I., Korotkova E.V., Kuznetsova E.E., Mukhamedieva L.N., Begrov V.V., Pepelyaev Yu.V. Apparatura dlya provedeniya biologicheskikh eksperimentov s ulitkami na pilotiruyemykh orbital’nykh stantsiyakh. [Equipment for biological experiments with snails at manned space stations]. Aviakosm. i ekol. med. [Aerospace and environ. Med.]. 2010. V. 44. № 3. P. 61–64 (in Russian).
• Javelidze G. Identifier of Georgian terrestrial mollusks. Tbilisi. Publishing house of “Tbilisi University”. 1972. (In Georgian).
• Dogel V.A. Zoologiya bespozvonochnykh. Pod red. Polyanskogo Y.U. I. 7-ye izd., pererab. i dop. M. Izd-vo “Vyssh. shkola”. [Zoology of invertebrates]. Ed. Polyansky Yu.I. 7th ed., Revised. and add. M. Publishing house “Higher School”. 1981. 606 p. (in Russian).
• Zaitseva O.V. Strukturnaya organizatsiya sensornykh sistem ulitki. [Structural organization of snail sensory systems]. Zhurn. vyssh. nervn. deyat. [J. higher. nervous active]. 1992. V. 42. № 6. P. 1132–1149. (in Russian).
• Korotkova G.P. Regeneratsiya zhivotnykh. SPb. Izd-vo “S.- Peterburg. un-ta”, [Regeneration of animals]. SPb. Publishing house “St. Petersburg University”, 1997. 480 p. (in Russian).
• Sidelnikov A.P. Regeneratsiya glaznogo shchupal’tsa u gigantskoy afrikanskoy ulitki Achatina fulica. [Regeneration of the eye tentacle in the giant African snail Achatina fulica]. Izv. RAN. Ser. biol. [Izv. RAS. Ser. biol.]. 1991. № 5. P. 716–725 (in Russian).
• Sidelnikov A.P. Regeneratsiya glaznogo shchupal’tsa u ulitki Achatina fulica i yeye regulyatsiya. Avtoref. dis. kand. biol. nauk. Sankt-Peterburg. [Regeneration of the optic tentacle in the snail Аchatina fulica and its regulation]. [Abstract of the thesis. dis. cand. biol. sciences]. St. Petersburg. 1997. (in Russian).
• Sidelnikov A.P. Vliyaniye plotnosti populyatsii na rost i regeneratsionnuyu sposobnost' ulitki Achatina fulica. [Influence of population density on the growth and regeneration capacity of the snail Achatina fulica]. Izv. AN. Ser. biol. 2000. № 5. P. 525–532 (in Russian).
• Tartakovskaya O.S. Vliyaniye ekologicheskikh faktorov (plotnost' populyatsii, svetovoy rezhim) i vozrasta na regeneratsiyu perifericheskogo otdela zritel’nogo apparata bryukhonogogo mollyuska Achatina fulica. Dis. kand. biol. nauk. Kaliningrad. 2003. 138 p.
• Chernorizov A.M., Shekhter E.D., Arakelov G.G., Zimachev M.M. Vision of the grape snail: spectral sensitivity of the dark-adapted eye. Zurnal vusshei nervnoi deytelynosti [Journal. higher. nerve. active]. 1992. V. 42. Issue 6. P. 1150–1155.
• Shekhter Ye.D., Grechenko T.N. Dva tipa fotoretseptorov v akhromaticheskoy zritel’noy sisteme vinogradnoy ulitki. Eksperimental’naya psikhologiya. 2009. V. 2. № 2. P. 5–15. (in Russian).
• Shekhter M.S., Chernorizov A.M., Gadel’shina D.F. Modul’naya organizatsiya mekhanizmov akhromaticheskogo zreniya: ot cheloveka k prostym nervnym sistemam. Natsional’nyy psikhologicheskiy zhurnal. 2010. № 2 (4). S. 92–99.
• Berg E., von, Schneider G. Langsame Belichtungspotentiale des Auges von Helix pomatias L. Naturwissenschaften. 1967. 54 Jahrgang. H. 22. S. 591–592.
• Chase R., Kamil R. Morphology and odor sensitivity of regenerated snail tentacles. J. Neurobiol. 1983. V. 14. № 1. P. 43–50.
• Chernorizov A.M., Shekhter E.D., Arakelov G.G., Zimachev M.M. The Vision of the Snail: The Spectral Sensitivity of the Dark- Adapted Eye. Neurocsi. & Behav. Physiol. 1994. V. 24. № 1. P. 59–62.
• Flores S.V., de Iraldi A. On the regeneration of the eye in Helix aspersa and Cryptomphallus aspersa. Z. Zellforsch. 1973. V. 142. P. 63–68.
• Gillary H.L. The regenerating eye of Strombus: anatomy and electrophysiology. American zoologist. 1972. V. 12. № 4. P. 691.
• Gillary H. Electrical potentials from the regenerating eye of Strombus. J. exp. biol. 1983. V. 107. P. 293–310.
• Grigoryan E.N., Dvorochkin N., Poplinskaya V.A., Yousuf R., Radugina E.A., Almeida E.A. The effect of hypergravity on the lens, cornea and tail regeneration in Urodela. Acta Astronautica. 2017. V. 138. P. 423–433.
• Grigoryan E.N., Radugina E.A. Behavior of stem-like cells, precursors for tissue regeneration in Urodela, under conditions of microgravity. Stem Cells and Development. 2019. V. 28. № 7. P. 1–15. https://doi.org/10.1089/scd.2018.0220
• Mattson P. Regeneration. Bobbs-Merrill comp. inc. Indianapolis. 1976.