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Volume 31 #2

Contents

  1. AUDITORY ADAPTATION TO MOTION: INTERSENSORY EFFECTS
  2. ANALYSIS OF THE YARBUS’S CONCEPTIONS ON THE ROLE OF THE BLIND RETINA IN COLOR PERCEPTION
  3. THE FEEDING BEHAVIOR OF THE MALLARD (ANAS PLATYRHYNCHOS) IN THE DARKNESS
  4. RESOLUTION OF SOUND RIPPLED SPECTRUM AN DIFFERENT BANDWIDTHS BY HUMANS: ELECTROPHYSIOLOGICAL STUDY
  5. STEREO CORRESPONDENCE PROBLEMS IN TERMS OF LINEAR THEORY OF SPECTRAL STIMULUS FORMATION
  6. SCREEN-PRINTED ELECTRODES MODIFIED WITH PROTEIN HYDROGEL AND BACTERIAL CELLS AS THE BASIS OF AMPEROMETRIC BIOSENSOR

SCREEN-PRINTED ELECTRODES MODIFIED WITH PROTEIN HYDROGEL AND BACTERIAL CELLS AS THE BASIS OF AMPEROMETRIC BIOSENSOR

© 2017 S. S. Kamanin, V. A. Arlyapov, O. N. Ponamoreva, I. V. Blokhin, V. A. Alferov, A. N. Reshetilov

Federal state budgetary educational institution of higher education “Tula state university”, 300012 Tula, Lenin av., 92
Federal state budgetary educational institution of higher vocational education “Tula state pedagogical university named after L.N. Tolstoy”, 300026 Tula, Lenin av., 125
Federal state budgetary educational institution of science G.K. Skryabin Institute of biochemistry and physiology of microorganisms, RAS, 142290 Moscow region, Pushchino, Nauka pr., 5

Received 20 Dec 2016

The electrodes, produced using matrix printing technique, were modified with protein hydrogel containing bacterial cells. The aim of this study was to estimate modification parameters and the effciency of biosensors during express- determination of biological oxygen demand (BOD). Protein hydrogel was formed from cross-linked bovine serum albumin covalently bound to residual ferrocene carboxaldehyde. Bacterial cells G. oxydans (ВКМ B-1280) were used as raw material for biocatalyst. For the mediators of ferrocene series: ferrocene, ferrocene carboxaldehyde, 1,1’-ferrocenedimethanol, 1,1’-dimethylferrocene or ethylferrocene the indices of their bioelectrocatalytic effciency were first determined in the course of immobilization in hydrogel. It was found that the mechanism of electron transfer in the system follows a ping- pong model. The use of protein hydrogel led to a considerable increase in stability of the analytical signal of the microbial biosensor. The BOD levels of wastes were detected using a fabricated biosensor. The range of measured BOD concentrations was 0.162–23 mg O2/l with the standard deviation of 5%. It was shown that the use of protein matrix based on ferrocene carboxaldehyde and the mentioned bacterial cells in combination with the electrode, produced through a matrix printing, provides the possibility of obtaining data strongly correlated with the results of standard method.

Key words: biosensor, screen-printed electrode, redox polymer, ferrocene derivative, G. oxydans

Cite: Kamanin S. S., Arlyapov V. A., Ponamoreva O. N., Blokhin I. V., Alferov V. A., Reshetilov A. N. Grafitovye pechatnye elektrody, modifitsirovannye provodyashchim belkovym gidrogelem i bakterialnymi kletkami, kak osnova amperometricheskogo biosensora [Screen-printed electrodes modified with protein hydrogel and bacterial cells as the basis of amperometric biosensor]. Sensornye sistemy [Sensory systems]. 2017. V. 31(2). P. 161-171 (in Russian).

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