Electrochemical biosensor based on Gluconobacter oxydans cells immobilized on the thermally expanded graphite (TRG) thin
lm of the electrode surface was developed for the sensibility enhancement of the ethanol detection. Сhronoamperometric
and cyclic current-voltage characteristics were obtained. Screen-printed electrodes (SPE) modi ed by TRG had higher
values of recorded currents in contrast to non-modi ed electrodes. Method of electrochemical impedance spectroscopy was
used for the study of electrical resistances and capacitances of SPE. It was shown that the increase of modi ed
electrodes signal amplitude was connected to the reducing of overall internal resistance of the system. A 3-fold
enhancement of the modi ed bioelectrode sensitivity to the ethanol was obtained compared to the value observed with an
unmodi ed biosensor.
Key words:
Gluconobacter oxydans, thermally expanded graphite, screen-printed electrodes
Cite:
Reshetilov A. N., Kitova A. E., Machulin A. V., Tarasov S. E., Gutorov M. A., Alferov S. V., Kolesov V. V., Gotovtsev P. M., Vasilov R. G.
Biosensor na osnove kletok gluconobacter i termorasshirennogo grafita
[Gluconobacter cells and thermally expanded graphite based biosensor].
Sensornye sistemy [Sensory systems].
2016.
V. 30(4).
P. 351-354 (in Russian).
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