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Involvement of protein kinase C in receptor-mediated signaling processes

© 2019 V. A. Penniyaynen, V. B. Plakhova, I. V. Rogachevsky, S. G. Terekhin, S. A. Podzorova, B. V. Krylov

Pavlov Institute of Physiology of Russian Academy of Sciences, 199034 St. Petersburg, Makarova emb., 6, Russia

Received 19 Feb 2019

Comenic acid is a specific agonist of opioid-like receptors found in the sensory neuron membrane. These receptors are coupled to NaV1.8 channels responsible for nociceptive information coding. Patch-clamp data show that at the membrane level, the ligand-receptor binding of comenic acid is manifested in a decrease in the effective charge transfer of NaV1.8 channels activation gating device. The use of a specific protein kinase C inhibitor (Tamoxifen) did not affect this receptor-activated mechanism. A completely different result is obtained at the tissue level: Tamoxifen totally blocks the signal triggered by comenic acid and directed radially to the nerve cell genome. Elimination of the neurite- stimulating effect of comenic acid by Tamoxifen demonstrates that protein kinase C participates in intracellular cascade processes under study as a sequential unit.

Key words: comenic acid, nociception, sensory neurons, patch-clamp method, organotypic nerve tissue culture method, NaV1.8 channels, protein kinase C

DOI: 10.1134/S0235009219030089

Cite: Penniyaynen V. A., Plakhova V. B., Rogachevsky I. V., Terekhin S. G., Podzorova S. A., Krylov B. V. Uchastie proteinkinazy s v retseptor-oposredovannykh signalnykh protsessakh [Involvement of protein kinase c in receptor-mediated signaling processes]. Sensornye sistemy [Sensory systems]. 2019. V. 33(3). P. 204-211 (in Russian). doi: 10.1134/S0235009219030089


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