Volume 31 #3

Contents

1. Development of functional impairments in the lateral geniculate nucleus of di erent hemispheres following early monocular deprivation
2. Eye optics and retinal topography in the Eurasian beaver Castor fiber L.
3. Recognition of projectively transformed planar figures. X. Methods for finding an octet of invariant points of an oval contour – the result of introducing a developed theory into the schemes of oval description
4. Mechanism of oxidative action of light on lipofuscin granules from the retinal pigment epithelium cells of the human eye: the role of superoxide radicals
5. Spatial and temporal integration in the chain of the transduction of olfactory signals
6. Adaptive image brightness stabilization for the industrial system of large moving object recognition
7. Fast binary linear clustering algorithm for small dimensional histograms

Comparative effect of visible light and superoxide radicals on the lipofuscin granules (LG) uorescent characteristics of the human eye retinal pigment epithelium (RPE) and their main fluorophore A2E was studied using methods of fluorescence spectroscopy and high performance liquid chromatography. It has been shown that both the irradiation with visible light and the action of superoxide radicals produced the increase of fluorescence intensity LG in the short- wavelength region of the spectrum (400–500 nm). At the same time light caused a decrease in fluorescence intensity LG in the long-wavelength region of the spectrum (500–600 nm) unlike superoxide radicals. Similar effects of visible light and superoxide radicals were also demonstrated with regards of A2E cardiolipin-containing liposomes and the chloroform extracts LG. These results suggest that visible light causes the more wide range of changes in the fluorescent properties of fluorophores LG from RPE cells than superoxide radicals in dark conditions. We conclude that the oxidative effect of light on LG is mediated not only superoxide radicals but also other reactive oxygen species generated during the photo-oxidative degradation of fluorophores of LG.

Key words: retinal pigment epithelium, lipofuscin granules, fluorophores, A2E, superoxide, derivatives of all-trans-retinal, fluorescence.

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