Mechanism of oxidative action of light on lipofuscin granules from the retinal pigment epithelium cells of the human eye: the role of superoxide radicals

© 2017 M.A. Yakovleva1, A.E. Dontsov1, N.L. Sakina1, P.M. Arbukhanova2, T.B. Feldman1,3, S.A. Borzenok2, M.A. Ostrovsky1,3

1Emanuel Institute of Biochemical Physics RUS, 119334 Moscow, Kosygin st., 4
2Sv. Fyodorov Eye Microsurgery Complex, 127486 Moscow, Beskudnikovsky bld., 59a
3Lomonosov Moscow State University, 119991 Moscow, Leninskie Gory, 1

Received 01 Mar 2017

Comparative effect of visible light and superoxide radicals on the lipofuscin granules (LG) uorescent characteristics of the human eye retinal pigment epithelium (RPE) and their main fluorophore A2E was studied using methods of fluorescence spectroscopy and high performance liquid chromatography. It has been shown that both the irradiation with visible light and the action of superoxide radicals produced the increase of fluorescence intensity LG in the short- wavelength region of the spectrum (400–500 nm). At the same time light caused a decrease in fluorescence intensity LG in the long-wavelength region of the spectrum (500–600 nm) unlike superoxide radicals. Similar effects of visible light and superoxide radicals were also demonstrated with regards of A2E cardiolipin-containing liposomes and the chloroform extracts LG. These results suggest that visible light causes the more wide range of changes in the fluorescent properties of fluorophores LG from RPE cells than superoxide radicals in dark conditions. We conclude that the oxidative effect of light on LG is mediated not only superoxide radicals but also other reactive oxygen species generated during the photo-oxidative degradation of fluorophores of LG.

Key words: retinal pigment epithelium, lipofuscin granules, fluorophores, A2E, superoxide, derivatives of all-trans-retinal, fluorescence.

Cite: Yakovleva M. A., Dontsov A. E., Sakina N. L., Arbukhanova P. M., Feldman T. B., Borzenok S. A., Ostrovsky M. A.. Mekhanizmy okislitelnogo deistviya sveta na lipofustsinovye granuly iz kletok retinalnogo pigmentnogo epiteliya glaza cheloveka: rol superoksidnykh radikalov [Mechanism of oxidative action of light on lipofuscin granules from the retinal pigment epithelium cells of the human eye: the role of superoxide radicals]. Sensornye sistemy [Sensory systems]. 2017. V. 31(3). P. 227-236 (in Russian).

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