Abnormal band in the circular dichroism spectrum of DNA cholesteric liquid-crystalline dispersions – an analytical criteriom for detection of coloured biologically active compounds

© 2016 Yu. M. Evdokimov, S. V. Semenov, S. G. Skuridin, V. I. Salyanov, P. N. Sol’ev, V. T. Valuev-Elliston, F. V. Vereshchagin, D. P. Chulkov, O. N. Kompanets

Engelgardt Institute of Molecular Biology, RAS Vavilov st. 32, 119991 Moscow
National Research Centre “Kurchatov Institute”, Kurchatov sq. 1, 123182 Moscow
Institute of Spectroscopy, RAS Fizicheskaya st. 5, Troitsk, 142190 Moscow

Received 30 Mar 2016

The сircular dichroism (CD) spectra of the cholesteric liquid-crystalline dispersions (CLCD) formed by double-stranded DNA molecules at various parameters of these particles (size, cholesteric pitch, etc.) have been calculated. Besides, the CD spectra in the case of colored antibiotics (analytes) interaction with DNA molecules in the content of dispersion particles were calculated. It was shown that an abnormal band in the CD spectra represents, by itself, a simple, convenient criterion, which allows one not only detect the presence of analytes in solutions being analyzed, but evaluate their concentration. It opens a possibility for the using CLCD particles as the biosensing units for analytical systems dealing with detection of colored antibiotics.

Key words: cholesteric liquid-crystalline dispersions, circular dichroism, abnormal optical activity, “quasinematic” layers, antibiotics

Cite: Yu. M. Evdokimov, Semenov S. V., Skuridin S. G., Salyanov V. I., Sol’ev P. N., Valuev-Elliston V. T., Vereshchagin F. V., Chulkov D. P., Kompanets O. N.. Anomalnaya polosa v spektre krugovogo dikhroizma kholestericheskikh zhidkokristallicheskikh dispersii dnk – analiticheskii kriterii dlya obnaruzheniya okrashennykh biologicheski aktivnykh soedinenii [Abnormal band in the circular dichroism spectrum of dna cholesteric liquid-crystalline dispersions – an analytical criteriom for detection of coloured biologically active compounds]. Sensornye sistemy [Sensory systems]. 2016. V. 30(3). P. 249-259 (in Russian).

References:

  • Belyakov V.A., Demikhov E.I., Dmitrienko V.E., Dolganov V.K. Optical activity, transmission spectra, and structure of blue phases of liquid crystals // Sov. Phys. JETP. 1985. V. 89 (6). P. 2035–2051 [in Russian].
  • Belyakov V.A., Sonin A.S. Optics of cholesteric liquid crystals. Moscow: Nauka, 1982. 360 p. [in Russian].
  • Yevdokimov Yu.M., Salyanov V.I., Savvateev M.N., Dubinskaya V.A., S.G. Skuridin. The study of “rigid” nanoconstructions formed by DNA liquid-crystalline dispersion particles using atomic force microscopy // Living Systems Technologies. 2013. V. 10 (1). P. 20–27 [in Russian].
  • Papkov S.P., Kulichikhin V.G. The liquid crystalline state of polymers. Moscow: Khimiya, 1977. 240 p. [in Russian].
  • Semenov S.V., Yevdokimov Yu.M. Circular dichroism of DNA liquid-crystalline dispersion particles // Biophysics. 2015. V. 60 (2). P. 242–252 [in Russian].
  • Chilaya G.S., Lisetskii L.N. Helical twist in cholesteric mesophases // Sov. Phys. Usp. 1981. V. 134 (2). P. 279–304 [in Russian].
  • Adamczyk A. Phase transition in freely suspended smectic droplets. Cotton-Mouton technique, architecture of droplets and formation nematoids // Mol. Cryst. Liq. Cryst. 1989. V. 170 (1). P. 53–69.
  • Belyakov V.A., Orlov V.P., Semenov S.V., Skuridin S.G., Yevdokimov Yu.M. Comparison of calculated and observed CD spectra of liquid crystalline dispersions formed from double-stranded DNA and from DNA complexes with coloured compounds // Liquid Cryst. 1996. V. 20 (6). P. 777–784.
  • Bouligand Y., Norris V. Chromosome separation and segregation in dinoflagellates and bacteria may depend on liquid crystalline states // Biochimie. 2001. V. 83 (2). P. 187–192.
  • Chiccoli C., Pasini P., Semeria F., Zannoni C. Computer simulations of nematic droplets with toroidal boundary conditions // Mol. Cryst. Liq.Cryst. 1992. V. 221 (1). P. 19–28.
  • Durand D., Doucet J., Livolant F. A study of the structure of highly concentrated phases of DNA by X-ray diffraction // J. Phys. II (France). 1992. V. 2 (9). P. 1769–1783.
  • Goldar A, Thomson H, Seddon J.M. Structure of DNA cholesteric spherulitic droplet dispersions // J. Phys. Condens. Matter. 2007. V. 20 (3). P. 035102 (1–9).
  • Gottarelli G., Spada G.P. Application of CD to the study of some cholesteric mesophases // Circular dichroism: principles and applications / Eds. Nakanishi K., Berova N., Woody R.W. New-York: VCH, 1994. P. 105–119.
  • Kornyshev A.A., Leikin S., Malinin S.V. Chiral electrostatic interaction and cholesteric liquid crystals of DNA // Eur. Phys. J. E. 2002. V. 7 (1). P. 83–93.
  • Leforestier A., Livolant F. Distortion of DNA cholesteric liquid crystal quenched at low temperature: geometrical
  • analysis and models // J. Phys. II (France). 1992. V. 2 (10). P. 1853–1880.
  • Lerman L.S. A transition to a compact form of DNA in polymer solutions // Proc. Natl. Acad. Sci. USA. 1971. V. 68(8). P. 1886–1890.
  • Lerman L.S. Chromosomal analogues: long-range order in ψ-condensed DNA // Cold Spring Harb. Symp. Quant. Biol. 1974. V. 38. P. 59–73.
  • Livolant F, Leforestier A. Condensed phases of DNA: structures and phase transitions // Prog. Polym. Sci. 1996. V. 21 (6). P. 1115–1164.
  • Nguyen T.T. Computational study of DNA condensation with divalent counterions, role of counterion size. arXiv:1508.02085v1 [q-bio.BM] 9 Aug 2015.
  • Norden B. Applications of linear dichroism spectroscopy // Appl. Spectrosc. Rev. 1978. V. 14 (2). P. 157–248.
  • Stanley C.B., Hong H., Strey H.H. DNA cholesteric pitch as a function of density and ionic strength // Biophys. J. 2005. V. 89 (4). P. 2552–2557.
  • Yevdokimov Yu.M., Salyanov V.I., Semenov S.V., Skuridin S.G. DNA liquid-crystalline dispersions and nanoconstructions. Boca Raton–London–New York: CRC Press (Taylor & Francis Group), 2011a. 258 p.
  • Yevdokimov Yu.M., Salyanov V.I., Skuridin S.G., Semenov S.V., Kompanets O.N. The CD spectra of doublestranded DNA liquid-crystalline dispersions. New York: Nova Science Publishers Inc, 2011b. 103 p.
  • Yevdokimov Yu.M., Skuridin S.G., Lortkipanidze G.B.
  • Liquid-crystalline dispersions of nucleic acids // Liquid Cryst. 1992. V. 12 (1). P. 1–16.
  • Yevdokimov Yu.M., Skuridin S.G., Salyanov V.I. The liquidcrystalline phases of double-stranded nucleic acids in vitro and in vivo // Liquid Cryst. 1988. V. 3 (11). P. 1443–1459.